leishtag.org
TrypTag is a genome-wide database of the subcellular protein localisation in the unicellular parasite Trypanosoma brucei . The main paper describing this resource is Billington et al. , 2023 .
Proteins were fluorescently labelled using endogenous tagging with the fluorescent protein mNeonGreen ( Shaner et al. , 2013 ) then the tagged cell lines were viewed by diffraction-limited widefield fluorescence microscopy. All tagging was done in the procyclic form.
Wherever possible, we have determine the localisation of the protein by N and C terminal tagging. N terminal tagging of proteins with a predicted signal peptide was avoided, and bear in mind that tagging may disrupt a targeting sequence. The localisation observed for each cell line has been manually annotated using a standardised ontology.
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