Genome surveillance during DNA replication in human health and disease
Accurate and timely propagation of genetic information across multiple generations of dividing cells is an essential process initiated by DNA replication and completed by segregation of intact and duplicated copies of the genome. The process of DNA replication relies on the precisely coordinated action of numerous proteins with complementary enzymatic activities of the ‘replisome’, a powerful molecular machine responsible for unwinding the parental DNA template and synthesizing nascent DNA. However, plethor
In our recently established lab, we are interested in exploring the molecular mechanisms that allow mammalian cells to fully and faithfully replicate their DNA content, repair their broken DNA, and align physiological cell cycle states to continuously varying cell metabolic conditions. To this end, we combine classical cell biology, biochemistry, genetics and quantitative mass spectrometry with state-of-the-art light imaging methodologies such as automated high-content microscopy and AI-assisted image analy